원문정보
Analysis of Angiotensin I Converting Enzyme Inhibitory Activity of Oligosacchride Extracted from Capsosiphon fulvescens
초록
영어
The hydrolysates prepared with various enzyme digestion of Capsosiphon fulvescens were used to measure the inhibitory effects against angiotensin I converting enzyme (ACE). The commercially available enzymes such as Celluclast, Viscozyme, Lysing enzyme, Flavourzyme, Alcalase and Pectinex were used to digest C. fulvescens and produce hydrolysates. The maximum ACE inhibitory activity was observed using Alcalase hydrolysis (72.9%). The optimal conditions of Alcalase extraction were pH 8.0 and extraction time for 12 hr. The hydrolysates were fractionated using preparative- LC and anion-exchange chromatography on DEAEcellulose and the fraction B and B-2 were isolated. The ACE inhibitory activity of fraction B-2 by anion-exchange chromatography was 82.6%. The molecular weight of fraction B-2 estimated using size exclusion chromatography was about 1 kDa. The monosaccharide composition of the fraction B-2 was determined to be mannose (1.1%), glucuronic acid (1.3%), galactose (1.3%) and glucose (96.3%).
목차
1. 서론
2. 재료 및 방법
2.1. 실험 재료
2.2. 산, 알칼리, 열수 및 유기용매 추출물의 제조
2.3. 효소 가수분해물 제조
2.4. 최적 추출조건 측정
2.5. 분취용 크로마토그래피를 이용한 올리고당 분리
2.6. Anion exchange chromatography를 이용한 올리고당 분리
2.7. HPLC에 의한 분자량 측정
2.8. 구성당 성분분석
2.9. ACE 저해 활성 측정
2.10. 통계처리
3. 결과 및 고찰
3.1. 추출 수율
3.2. 매생이 추출물의 ACE 저해활성
3.3. 최적 추출조건 측정
3.4. 매생이 유래 올리고당 분리
3.5. 올리고당의 분자량 측정
3.6. 구성당 성분분석
4. 결론
REFERENCES