원문정보
Effect of Pine needle Ethanol Extracts on the Inhibitory Activity of Atopic Dermatitis
초록
영어
The aim of this study was to examine inhibitory effects of pine needle ethanol extracts (PNEE) on atopic dermatitis (AD). To determine inflammatory activity PNEE was added to LPS-induced murine peritoneal macrophages for an in-vitro test. In addition, anti-AD test was carried out by spreading PNEE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)- induced BALB/c mice. It was confirmed that the nitric oxide (NO) secretion was suppressed when 1~ 50 μg/mL of PNEE were added to LPS-induced murine peritoneal macrophages. Moreover, levels of TNF-α, IL-6, and IL-1β‚ were decreased. For the anti-AD test, PNEE alleviated symptoms of the erythema in DNCB-induced mice. Furthermore, the IFN-γ secretion of the group treated with PNEE was increased in splenocytes from DNCB-induced mice compared to the positive control, while IL-4 secretion diminished. Through these results, we can conclude that PNEE can inhibit AD by modulating the IFN-γ, IL-4 cytokines production and inhibiting inflammation.
목차
1. 서론
2. 재료 및 방법
2.1. 재료
2.2. 실험동물
2.3. pH 및 색도
2.4. 대식 세포 분리 및 배양
2.5. 대식 세포의 nitric oxide 분비량 측정
2.6. 대식 세포의 cytokine 분비량 측정
2.7. 아토피 피부염 유발 및 시료 처리
2.8. 비장 세포 분리 및 배양
2.9. 비장 세포 배양액의 cytokine 분비량 측정
2.10. 혈청의 total IgE 함량 측정
2.11. 비장 세포의 증식능 측정
2.12. 통계 처리
3. 결과 및 고찰
3.1. pH 및 색도
3.2. 대식 세포의 nitric oxide 분비량
3.3. 대식 세포의 cytokine 분비량
3.4. 비장 세포 배양액의 cytokine 분비량
3.5. 혈정의 total IgE 함량
3.6. 비장 세포의 증식능 측정
3.7. 아토피 피부염 증상
4. 결론
감사
REFERENCES