콜라겐 합성과 MMP-1 발현에 대한 생물전환 지실 추출물의 효과


Effect of Ponciri Fructus Extracts Fermented with Ganoderma lucidum on the Collagen Synthesis and Expression of Matrix Metalloproteinase-1

이계원, 박성민, 유영춘, 조영호

피인용수 : 0(자료제공 : 네이버학술정보)



Ponciri fructus, the unripe fruits of Poncirus trifoliata, are widely used in oriental traditional medicine as a remedy for inflammation, gastritis, emesis, digestive ulcers, allergy, and dysentery. To study the anti-wrinkle effects of Ponciri fructus extract (PFE) containing flavanone glycosides, PFE was fermented with Ganoderma lucidum mycelia and its biological activities were investigated. In Ponciri fructus extracts fermented with G. lucidum (G-PFE), polyphenol content was 1,021.00±0.50 μg/mL and flavonoid content was 589.41±0.21 μg/mL. G-PFE was found to scavenge 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radicals and superoxide anion radical by a dose dependent manner, respectively. G-PFE showed higher antioxidant activity than that of PFE. In addition, the photoprotective properties of G-PFE was tested in human dermal fibroblasts (HDF) exposed to UVA radiation. G-PFE inhibited the activity of matrix metalloproteinase-1 (MMP-1) and showed a dose dependent decrease in the expression level of MMP-1. G-PFE also increased collagen biosynthesis in HDF. These results demonstrate that G-PFE could be useful as a potential cosmetic ingredient for anti-wrinkle.


 1. 서론
 2. 재료 및 방법
  2.1. 공시 균주, 세포 배양 및 시약
  2.2. 지실 추출물 제조
  2.3. 생물전환 지실 추출물 제조
  2.4. β-glucosidase 활성 측정
  2.5. 생물전환 지실 추출물의 유효성분 확인
  2.6. 항산화 활성
  2.7. 세포독성 측정
  2.8. 콜라겐 생합성 촉진 효과 측정
  2.9. MMP-1 활성 저해 효과 측정
  2.10. MMP-1 발현 저해 효과 측정
  2.11. 자료분석 및 통계처리
 3. 결과 및 고찰
  3.1. 총 폴리페놀 함량 및 플라보노이드 함량 측정 결과
  3.2. HPLC를 이용한 flavanone aglycone 함량 변화 분석 결과
  3.3. DPPH 라디칼 소거 효과
  3.4. Superoxide 음이온 라디칼 소거 효과
  3.5. 세포 독성
  3.6. 콜라겐 생합성 촉진 효과
  3.7. MMP-1 활성 저해 효과
  3.8. MMP-1 발현 저해 효과
 4. 결론


  • 이계원 Gye Won Lee. 건양대학교 제약생명공학과
  • 박성민 Sung Min Park. (주)코씨드바이오팜 기술연구소
  • 유영춘 Yung Choon Yoo. 건양대학교 의학과
  • 조영호 Young Ho Cho. 건양대학교 제약생명공학과


자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.