earticle

영어

In this study, we evaluated the effects of solvent system on the increased surface area precipitation process for the purification of paclitaxel from plant cell cultures of Taxus chinensis. The purity of paclitaxel after 24 hr of acetone/pentane precipitation was 54.0% when there was no surface area increase, while it was 77.7% when the surface area was increased by the use of anion exchange resin (Amberlite IRA-400OH). The purity of the precipitate was relatively higher, presumably because a considerable portion of the impurities as well as paclitaxel was adsorbed to the ion exchange resin. However, the yield of paclitaxel decreased when Amberlite IRA-400OH was used. These results are in contrast with the results of the methanol/water precipitation process. From these results, it was found that the precipitation behavior varies considerably depending on the use of the solvent system (methane/water or acetone/pentane). Compared with the case where no surface area increasing agent was employed, the addition of Amberlite IRA-400OH as a surface area increasing agent resulted in a considerable decrease in the size of the paclitaxel precipitate. When Amberlite IRA-400OH was added, the zeta potential value in the precipitation solution was higher (zeta potential value: -21.36 mV) than when no surface area increasing agent was employed (zeta potential value: -0.52 mV), indicating that the precipitated particles are much more stabilized with the addition of Amberlite IRA-400OH.