원문정보
초록
영어
Gene therapy is a therapeutic method to treat human disease by modifying the genes of cells. One of the important criteria of a successful gene therapy is the efficiency of gene delivery with safety. Recently, lots of protocols have been developed for gene delivery systems using viral and non-viral vectors. However, it may have limitations in the stimulation of stem cells due to its low transfection efficiencies.In this study, we developed substrate-mediated viral gene delivery system with adhesive materials (catecholamine polymers) using for macro-patterned gene delivery by “pipet drawing”. The sticky adeno-associated viral vector is formulated with catecholamines, which is specialized adhesive proteins of marine mussels "mytilus edulis". Catecholamine polymers such as PEI-catechol, polydopa were used to functionalize surfaces of AAV to spatially controlled gene expressions. We used sticky viruses for patterned gene delivery system to various cell lines especially human neural stem cells. Importantly, a simple and versatile approach to spatio-controlled gene delivery in human neural stem cells was demonstrated using pipette drawing. Development of gene delivery system to stem cells has an importance in the gene therapy and tissue engineering applications, due to its enormous potential to threat many diseases which have no effective therapy until now.