earticle

영어

Synthetic biology devices have additionally been developed to serve as biomedical tools. The rational and model-guided construction of biological parts enables a new way to develop therapeutic platforms. Here we present a novel genetic circuit strategy that is designed to induce the constitutive expression of anti-leukemia drug by using the ligand-independent activity of a transcription factor. For this purpose, we first constructed a functional transcription factor (AraCc) by rationale design, which is no longer required an inducer to bind its own promoter PBAD. Then we established a novel circuit that operated by self-positive feedback activity of AraCc. This system can generate target gene transcription persistently when the initial expression of AraCc is triggered. Such synthetic circuits could facilitate the delivery of drugs into the target cells in favorable condition because it eventually set a stage for synchronizing cells memorized by auto-activating transcription factor.