원문정보
초록
영어
OCA2 or P-protein (pink-eyed dilution protein) plays a critical role in melanin synthesis in retinal pigment epithelium (RPE) and melanocytes cells. Thus mutation in this protein induces a complete or partial albinism. The aim of the study was to compare the shape, size and count of melanosomes in wild and transfected melan-a by SiRNA (siRNA for OCA2 mRNA) and compared to melan-p1. The above cells were examined by In vitro DOPA staining, and the melanosomes were characterized by the Transmission Electron Microscope (TEM). The results suggest significant changes in melanosomal morphology and their respective numbers. It was also studied to find out the role in trafficking of melanosome by co-culture method. These results suggested that the P-protein can control the synthesis melanin as well as melanosome biology. Thus, we anticipate the role of P-protein in skin pigmentation can be explored for the development of new class of cosmetics.