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As a traditional oriental medicine, ginseng has been used for thousands of years. And the ginseng seed oil has been used in cosmetics. However, little is known about its cosmeceutical efficacies. This study was conducted to evaluate the inhibitory effects of ginseng seed oil extracted by supercritical carbon dioxide (SC-CO2) on the production of NO in LPS-activated Raw 264.7 cells. In activated macrophage, large amounts of nitric oxide (NO) are generated by inducible nitric oxide synthase (iNOS), resulting in acute or chronic inflammatory disorders. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, using Griess reagent to measure the NO generated by LPS-activated Raw 264.7 cells in DMEM without phenol red. Compare with blank 10ppm Ginseng seed oil extracted by SC-CO2 can inhibit 50% nitric oxide (NO) to generate. Cell viability was determined by MTT assay, and the ginseng seed oil extracted by SC-CO2 had no cytotoxicity during the entire experimental period. As we know skin inflammation along with dryness, in the Moisturizing ability test in vivo showed that ginseng seed oil’s moisturizing ability 3% higher than 100% BG, and 10% higher than blank. Transepidermal water loss (TEWL) of Ginseng seed oil extracted by SC-CO2 was measured in vitro showed that Ginseng seed oil can boost the skin’s ability to retain moisture. This study showed that ginseng seed oil was an excellent moisturizing and anti-inflammation ability.