원문정보
초록
영어
Two-component signal transduction systems, which consist of a histidine sensor kinase and a response regulator, are gaining increasing interests as a useful bioparts to construct artificial genetic regulatory circuits for synthetic biology. In the present study, we investigated the possibility to use a heterologous two-component signal transduction system to fabricate an E. coli biosensor. First, we constructed an inducible expression vector containing todS sensor kinase and todT response regulator genes of the aromatic compounds-sensing TodST system of Pseudomonas putida. To use as a reporter strain,a transcriptional fusion between the TodT regulatable promoter of P. putidatodX gene and beta-galactosidase gene was then integrated into the chromosome of an E. coli strain. The beta-galactosidase activity of the reporter strain harboring TodST expression vector was strongly induced by TodST in the presence of various aromatic compounds, demonstrating the possibility to employ TodST system as a backbone for artificial aromatic compounds signal transduction system. Supported by the Intelligent Synthetic Biology Global Frontier Program, and the Next-Generation BioGreen 21 Program.