earticle

영어

In this study, a fully integrated and portable Rotary Genetic Analyzer has been developed to analyze the gene expression of influenza A virus. The Rotary Genetic Analyzer is made up of four parts: a disposable microchip, three thermal blocks for temperature control, a servo-motor for precise and high-rate spinning of the chip, and a miniaturized optical fluorescence detector. The microdevice was designed for performing microglass bead-based RNA template purification and then Rotary reverse transcriptase polymerase chain reaction (RT-PCR). Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.