원문정보
초록
영어
The intestinal microbiome of animals is composed of a diverse and complex community of bacteria. Because of its impact on physiological, developmental, nutritional, and immunological processes, the microbial community of animals markedly influences health status and performance of the animal. Thus, in order to verify the effect of probiotics and of other feeding materials, which are likely to influence the composition of the intestinal Microbiome and to explain the mechanisms of their actions, it is essential to develop methodological strategies and useful tools to characterize the microbiome of animals. For the assessment of health status of swine, we prepared fosmid clone libraries from swine guts of different status of health and selected fosmid clone candidates which potentially probe swine health status. For selection of fosmid clone probes, metagenomic DNA from swine gut was hybridized to fosmid clone library. From hybridization result, candidate probes were selected. Origin of the selected probes were characterized based on end-sequencing of the fosmid clones. This work was supported by the MEST and NRF through the Human Resource Training Project for Regional Innovation