원문정보
초록
영어
Among the regulatory proteins of melanogenesis the Microphthalmiaassociated transcription factor (MITF), plays a key in expression of the essential protein in regulation of the melanin biosynthesis in melanocytes. For the transcription initiation of the mRNAs responsible for translation of melanogenesis associated proteins, the binding of the MITF to E-box is being required. The aim of the present study was to develop a SPR based assay for the evaluation of interaction of MITF with respective DNA binding site (E-box, CATGTG). A Label-free gold chip based DNA-protein interaction assay, being used to evaluate the interactions. The MITF showed almost a twice higher resonance signal after binding with E-box, when compared to mutated E-Box. We have also assessed various parameters to stabilize the assay conditions, to diminish the false signals. We anticipate that using this system a huge number of natural extracts may screen for the identification of potential depigmenting agents.