earticle

영어

Conventional point-of-care (POC) devices need to be more convenient because blood sampling and sample analysis are implemented in separate devices. Therefore, we have developed a one-step microfluidic POC device which can carry out blood sampling and sample analysis simultaneously on a device. The one-step microfluidic device has three components, a microneedle (an inner diameter: 80 μm) and a micropump for blood sampling and a microchannel for immuoassay. The micropump is a cylinder (dimameter: 9 mm, height: 15 mm) on a flexible thin film (1 mm tick) made of elastic polymer polydimethylsiloxane. The microchannel is fabricated with UV-curable polymer, Norland Optical Adhesive (NOA63), by contact printing method. Then, the surface of the channel was coated with PDMS nanolayer and modified to be hydrophilic by treating oxygen plasma. Finally, the microneedle and the micropump were assembled by bonding after oxygen plasma treatment and the microneedle was bonded to the side of the micropump. We observed the one-step microfluidic device could pump 300 ㎕ distilled water and blood mimicking solution as well. Then, the pumped sample solution was spontaneously flowed into the hydrophilic microchannel sequentially. Therefore, the one step microfluidic device can be used as a POC device for quick and convenient diagnosis on site.