earticle

영어

To prove behavior of cells in different cell cycle, cell synchronization has been used. It is arduous and time-consuming to confirm cell cycle arrest using fluorescence-assisted cell sorting (FACS) or Western blot. Electrochemical method has received attention as a label-free detection. We devise a label-free tool for in situ monitoring of cell cycle arrest using electrochemical method on PC12 cells. Redox reaction of cells is important because of showing behavior of cells in different phases. Results were validated by analyzing FACS and Western blot and signals of cell were detected by DPV. Consequently, our cell chip using electrochemical method can be useful tools in detecting cell cycle progression. Acknowledgements: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST)(2012-0000163) and by The Nano/Bio Science &Technology Program (M10536090001-05N3609-00110) of the Ministry of Education, Science and Technology (MEST) and by the Original Technology Research Program for Brain Science through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.(2012-0006592)