earticle

영어

Spectroscopic monitoring of proteins of interaction in live cells provides valuable information on function, interactions, and trafficking of proteins in their native context. Here, we describe a method to introduce spectroscopic probes to target proteins with a spatio-temporal control. We are utilizing split-intein mediated protein trans-splicing reaction for fluorescent labeling of target proteins. We also adopted a molecular switch to control the intein mediated reaction by using external stimuli.