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논문검색

Sustainability of Human Nerve Cell on New Carbonbased Materials

원문정보

Hong-Gi OH, Hyo-Geun NAM, Hye-Bin PARK, Si-Hyoung KIM, Da-Ae JO, Chang-Man KIM, Kwang-Soup SONG, Kwang-Hwan JHEE

한국생물공학회 한국생물공학회 학술대회 2013 춘계학술대회 2013.04 pp.300-300
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Graphene and diamond thin film have drawn the potential applications for biomedicine and biosensor due to their electrical properties, low background current, biocompatibility, wide potential window, and physical-chemical stability. Here, we report the cell culture conditions, which are exposed to graphene and diamond thin film using human nerve cell, SH-SY5Y. In order to confirm the cell viability and morphology, we adopt the assays of MTT, LDH, caspase-3/7 activity, cell adhesion, optical microscope image and fluorescence microscopy images stained with DAPI and Calcein AM. SH-SY5Y cells exhibited 70-95% viability on graphene-coated with various substrates such as glass, SiO2/Si, and polyethylene terephthalate (PET) compared to cell culture polystyrene (CCPS). Human nerve cell exhibited the 95% growth on diamond compared with CCPS after 3 days culturing. Fluorescence data exhibited the diamond thin film did not influence the shape of the cells in comparison to control plate. Our results showed that graphene and diamond thin film can be very good candidates for the application to the fabrication of biological sensor, biomolecules patterning, and study of cell-surface interactions.

키워드

저자정보

  • Hong-Gi OH Dept. of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, 730-701.
  • Hyo-Geun NAM Dept. of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, 730-701.
  • Hye-Bin PARK Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701.
  • Si-Hyoung KIM Dept. of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, 730-701.
  • Da-Ae JO Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701.
  • Chang-Man KIM Gumi Electronics & Information Technology Research Institute, Biomedical IT Convergence Center, Gumi, 730-701.
  • Kwang-Soup SONG Dept. of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi, 730-701.
  • Kwang-Hwan JHEE Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701.

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자료제공 : 네이버학술정보

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