earticle

영어

Recently, various biotechnological applications have been attempting by using DNA-based nano-technology. Chemical cross-linker has been widely applied in this field for the purpose of the DNA immobilization on the surface or DNA-Protein Crosslink (DPC). However, the chemical cross-linker (eg, EDC, DCC) has inevitable problems such as non-specific binding, by-products, multiple reaction steps. To overcome these disadvantages, a novel innovation approach is being considered with the concept, which introduces a reactive functional group at the end of DNA oligomer to achieve site-specific binding and controllable molecular orientation. In this study, we developed enzymatic preparation of reactive-end DNA oligomer via Terminal deoxynucleotidyl Transferase (TdT). As a reactive functional group, oxanine, which is one of the modified nucleobases, was incorporated into the end of DNA oligomer by TdT. It was found that initiator’s secondary structure and CoCl2 concentrations should be optimized for efficient incorporation of oxanine. The developed reactive-end DNA oligomer would be employed for fabrication of DNA-directed biomolecule array systems.