earticle

영어

Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. The promoter activity is a key factor in a cell for the active growth of living things and mass production of useful materials, but also abnormal growth of cancer cells. To investigate the promoter activity, interactions of RNA polymerase with their promoters have also been studied by a variety of biochemical methods, like a in-vivo and in-vitro transcription. Previous methods are time-consuming and complicated researches, so for the faster and easier monitoring of genetic information processing LSPR analysis used in this study. Using SP6 promoter-RNA polymerase as a model, different LSPR λmax shift were recorded with various promoter sequences induced by point mutation. As an outcome of this analysis, we can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This result successfully demonstrates a non labeling and simple analysis for promoter activity, and it possesses a great potential as a sensitive, on-chip and multiplexing gene analysis.