원문정보
초록
영어
The carotenoid pigment astaxanthin is strong antioxidants that have important applications in the pharmaceutical, cosmetics, food, and feed industries. Haematococcus pluvialis is known to be the richest source of natural astaxanthin. Generally, detection methods of astaxanthin call for extraction process of astaxanthin by organic solvents, incorporating breakdown processes of cyst cells such as mechanical disruption, spray drying, enzyme lysis, have been developed for the separation of astaxanthin from the cyst cell. Our results suggest that application of the lipidic dye Nile red for detection of lipophilic compound astaxanthin. By staining cells with Nile red, we can sense the low fluorescence of astaxanthin contents. In this work, Nile red was used as an indirect measurement of cellular astaxanthin contents. This method allowed the development of cytometric method faster than the traditional techniques for detection of astaxanthin in cyst cell.