원문정보
초록
영어
Bio-imprinting of Thermomyces lanuginosus lipases and selective adsorption of algal lipids by molecular imprinting polymer were performed. The extent of self-assembly of functional monomer-template complexes suggested a saturation equilibrium related with non-covalent bonding in constructing medium affinity recognition sites in the product polymer. Concerning that the greatest difference in relative concentrations corresponds to the minimum monomer groups randomly distributed relative to those involved in template complexation, the saturation isotherms was examined in the molar ratios employed, indicating site heterogeneity due to the distribution of non-complexing monomer throughout the polymer matrix. Bio-imprinting of sorbitan monooleate showed selective adsorption of algal lipids (neutral, glyco- and phospholipids), due to the guest-host complex after polymerization of methyl acrylate. Imprinting exhibited little influence on the enantioselectivity, while the specific recognition sites constructed depended on both the synergistic effect of hydrophobic interaction and shape complementary effect.