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영어

The growth of three marine green microalgae such as Dunaliella salina DCCBC2, Dunaliella tertiolecta and Dunaliella sp., were compared under 7 marine media. The algal cells were cultured in 500 mL flask with 300 mL of working volume and the culture conditions were followed as: temperature 25-26°C, light intensity 100 μmol/m2/s, and light cycle 12h:12h (light/dark). Among the seven different marine medium used in the present study, the highest biomass obtained in Spirulina and Zarrouk's medium was 0.726 g/l and 1.09 g/l (D. salina DCCBC2), 0.66 g/l and 0.76 g/l (D. tertiolecta) and 0.924 g/l and 0.76 g/l (Dunaliella sp.) respectively. The growth of all three marine algae was better in Spirulina and Zarrouk's medium than other media tested. This is mainly due to the presence of bicarbonate inorganic carbon source in Spirulina and Zarrouk's medium. The bicarbonate has high soluble nature than gaseous CO2 in the media and the marine algal species synthesis an important extracellular enzyme called carbonic anhydrase, which catalysis the inter-conversion of HCO3- and CO2-. Many microalgal species were actively uptake these compounds for their growth as well as lipid accumulation in the cells. In summary, the addition of inorganic bicarbonate carbon sources in the Spirulina and Zarrouk's medium showed highest microalgal growth and these two media are better for the cultivation of microalgae in industrial scale.