원문정보
초록
영어
Camphor is well known as an ingredient in Cinnamomum camphora (Camphor tree). Camphor ingredients have been used to treat skin inflammation, acne, oily skin, arthritis, muscle aches, rheumatism, bronchitis, coughs, colds, constipation or diarrhea as well as preservatives, pesticides, soaps, pesticides manufacturing, and spices. However, it was reported that the ingredients had neuro-toxicity and embryo-toxicity to patients and children. Therefore, in this experiment morphological toxicity of camphor to zebrafish embryo was investigated to apply it as a biocide. In addition, the influence of chloroform used as the solvent for camphor was examined under the same concentration level employed for toxic assessment of camphor. By observation results of embryo hatching, when the concentration of comphor and chloroform increased twice, hatching rate was 8.66% and 2.66%, respectively, indicating that hating toxicity by camphor was 3.3 times higher than that of chloroform. Morphological abnormality of 6.67% was observed in camphor of 1㎎/ℓ. In 72 hours hatched embryo without the head and tail was observed, and in 96 hours hatched embryo with bending somite and tail abnormality. Also, under the chloroform of 1㎎/ℓ, morphological abnormality of 10% was observed and at 96 hours hatched embryo with tail malformations and stopped heartbeat.