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Resistance of Lysinibacillus sphaericus WJ-6 Isolated from Concrete Pavement to Extreme Stresses

초록

영어

Concrete is the most used construction material worldwide. However during concrete production, large amount of CO2 is emitted in the atmosphere exerting harmful influence on the environment. Also, crack of concrete weakens the concrete construction durability and cause the corrosion. As one way to prevent this problem, self-healing concrete using calcite forming bacteria has been proposed. In this study, we isolated Lysinibaclillus sphaericus WJ-6 from concrete pavement then investigated sporulation yield and response of spore against various environmental stresses. Calcite of 10 mg/mL was determined by calcite dry weight measurement. When observed by microscopy, L. sphaericus WJ-6 showed spore formation and maximum spore yield was approximately 98.9%. Also response of spore against various environment stresses was examined. Spore was maintained approximately 83-97% survivability at heat (60℃), osmotic (3M), and alkaline (pH 12) stress. Base on this result, we will examine the potential of L. sphaericus WJ-6 to act as self-healing agent in concrete.

저자정보

  • Sang-Hyun HAN Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • Chang-Ho KANG Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • Jae-Ho CHOI Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • Dae-Yung KWAK Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • JunGu NOH Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • HanEul JEON Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • YuJin SHIN Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • SooJi OH Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
  • Jae-Seong SO Department of Biological Engineering, Inha University, Incheon 402-751, Korea.

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