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Dual Enzyme Activities of Microbacterium oxydans on Alginate and Laminarin

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영어

Recent breakthroughs in converting diverse carbohydrates from seaweed into renewable sources through metabolic engineering have demonstrated alternative methods, although relatively unexplored such as bioenergy source. Brown seaweed include almost 1800 species and are farmed extensively as food in China, Japan, and Korea. The composition of brown seaweed was included up to 55% of the carbohydrates like laminarin, mannitol and also contain alginate, which are structural polysaccharide in the cell wall. Laminarin can be easily hydrolyzed by laminarase to release glucose. However, the full potential of biofuel production from brown seaweed has not yet been realized because microorganism were not able to metabolize the alginate component. In this study, we newly isolated a microorganism, Microbacterium oxydans, from the soil, Its has ability, which be able to degrade laminarin and alginate. We analyzed characteristic decomposition by cell density, pH, reducing sugars, TLC and HPLC. Enzyme synthesis by Microbacterium oxydans showed unique characteristics, which were exolytic on laminarin and endolytic on alginate, respectly.

저자정보

  • Eun Jung KIM Dept. of Biotechnology, Pukyong National University, Busan.
  • Joong Kyun KIM Dept. of Biotechnology, Pukyong National University, Busan.

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