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영어

In Korea, ocean dumping of fishery wastes has been strictly prohibited after 2012 due to London convection of deep-sea dumping and effective reutilization of them has been being studied. Among the fishery wastes, 90 thousands tons of wastes are seaweeds. Green-seaweeds wastes contain lots of carbohydrate such as cellulose, pectin, xyloglucan and ulvan, so bacteria which have potential to degrade these polysaccharides to monosaccharides were focused for reutilization of renewable source. Potential bacteria were isolated from marshy soil and among them one strain was shown carboxymethyl-cellulose (CMC) degrading ability. It was identified Bacillus licheniformis with 98% similarity and named B.licheniformis TK3-Y. With 10% (v/v) inoculum size, optimization of the bacterium culture was carried out in 250 ml flasks (with 100 ml working volume) using 10 g/l CMC media for 4 days at various conditions. Cell density and concentration were measured by spectrophotometer at 600 nm and reducing sugar concentration was measured by DNS method at 540 nm. In optimum condition, the maximum reducing sugar yield was shown approximately 7.1%. It was also shown protein and lipid degrading abilities, which would be good to treat mixed-type fishery waste.