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Characteristics of Degradation Laminarin by Single and Mixed Microorganisms in 5 L Bioreactor

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During recent years, the amount of seaweed waste has increased due to its culture as an industrial resource and a depolluting plant for cleaning inland sea area, and eutrophication of seawater. The efficient treatment of seaweed waste is deemed necessary nowadays. In this study, six strains, designated EJ1-EJ6 were used, for reutilization of brown-seaweed waste. Among six strains, EJ4 showed the highest degradation ability, and its degradation ability was compared to that of the mixed culture on laminarin in a 5 L bioreactor using 20%(wt) of inoculums. In the experiment , 5 g/L of Eisenia bicyclis was used as a substrate due to its contains high content of laminarin in comparison with other brown seaweed. The mixed culture produced 1.13 g/l and EJ4 produced 1.86g/l of reducing sugars. The pH of mixed culture and EJ4 reached to 4.53 and 3.98, respectively. HPLC and TLC analyses showed that mixed culture produced less amounts of mono-, di-, tri- and oligosaccharides than single culture. These various types of saccharides would be applicationn to bioactive substance, fertilizer, bioethanol, etc.

저자정보

  • Eun Jung KIM Dept. of Biotechnology, Pukyong National University, Busan.
  • Da Som KANG Dept. of Biotechnology, Pukyong National University, Busan.
  • Joong Kyun KIM Dept. of Biotechnology, Pukyong National University, Busan.

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