원문정보
초록
영어
SS10 microbial strain, which is isolated from soil of sweet sorghum field, was characterized and identified as Staphylococcus sp. by analysis of 16S rDNA sequence and biochemical studies, and named as Staphylococcus sp. SS10 which has high xylanase activities. The optimum temperature and pH for xylanase activity of Staphylococcus sp. SS10 were 50℃ and 10.0, respectively. The xylanase activity was strongly inhibited by Al+++. The xylanase gene was cloned from Staphylococcus sp. SS10 genomic DNA by polymerase chain reaction (PCR). The amplified PCR product was ligated with the T&A cloning vector system and the constructed plasmids were transformed into E. coli DH5α. The sequence analysis of the insert DNAs revealed the identification of a 640-bp region containing xylanase open reading frame. According to xylanase gene sequence analysis, Staphylococcus sp. SS10 had gene sequence similarity of 99% with Bacillus subtilis Xyl gene for xylanase (AB457186.1).