원문정보
초록
영어
Research on the synthesis gas utilization for the production of fuels and chemicals has been the focus of researchers in the recent past. CO-utilizing bacterial strain Oscillibacter sp. C5, showing isovalerate production, was isolated from cow feces and characterized based on 16s rRNA gene sequence and physiological and phylogenetic properties. This strain indicated 99% of homology with Oscillibacter valericigenes Sjm18-20 T (DSM 18026T). This strain was strict anaerobic microorganism, rod shaped and non-motile microorganism. The cells were 0.4‒0.5 μm in width and approximately 2μm in length. Oscillibacter sp. C5 produced acetate and butyrate in culture broth using CO as energy and carbon source that confirms its acetogenic propetry which has acetyl-CoA pathway for the reductive synthesis of acetyl-coenzyme A (CoA) from H2/CO2 or CO, terminal electron-accepting, energy-generation process, and synthesis of cell carbon. Then, interesting point is the production of isovalerate (C-5 chemical) from CO, which has not been reported yet. The isovalerate is an important chemical which is used as a raw material for; plasticizer, lubricant, glue, powder coating, perfume and medicine. The aim of this study is to present the physiological and phylogenetic characteristics of newly isolated Oscillibacter sp. C5 along with GC-MS results confirming the isovalerate production.