earticle

영어

The waste from beer fermentation broth (WBFB) a semi solid biomass is an excellent resource for the production of bio-fuel and other biomaterials. WBFB supernatant was barely evaluated for the production of bioethanol without addition of any exogenous hydrolyzing enzymes, nutrients and microbial culture. Considering the fact, the current study was conducted to investigate the existence, origination and classification of various hydrolyzing enzymes involved in bioethanol production. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the existence of such hydrolyzing enzymes was confirmed in WBFB supernatant secreted from malt and yeast cell. Malt derived hydrolyzing enzymes were partially purified through series of purification step and examined their activity at each purification step using carboxymethyl cellulose as a substrate under various conditions. As the temperature increased gradually from 25 to 70°C, yeast cells in the chemically defined medium lost their cell wall and viability and released the cell matrix into the culture media at elevated temperature (45–70°C) were monitored through microscopic pictures. The presence of hydrolyzing enzymes from malt and glycolytic and fermentation enzymes of yeast in WBFB supernatant is expected to play a role in bioethanol production using simultaneous saccharification and fermentation without the need for additional enzymes, nutrients, or microbial cells via a cell-free enzyme system.