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논문검색

Development of CO2 capture biomimetic system using carbonic anhydrase as biocatalytic agent

원문정보

Seung Pil PACK, Bashistha Kumar KANTH, Ki Ha MIN, Young Ha RYU, Ki Baek YEO, Eui Kyung JANG, Mi-Ran KI

한국생물공학회 한국생물공학회 학술대회 2013 춘계학술대회 2013.04 pp.253-253
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Carbonic anhydrase (CA, EC 4.2.1.1) is one of the enzymes, which can be employed for CO2 sequestration technology development. CA catalyzes a reversible hydration of carbon dioxide: CO2 + H2O ↔ H+ + HCO3- (1.1) (Box 1) including varieties of other reactions. Interestingly, CA has the ability to catalyze the hydration of over 600,000 molecules of carbon dioxide per molecule of CA per second comparable to a theoretical maximum rate of 1,400,000. CA can fix large quantities of CO2 into CaCO3 in presence of suitable cations at modest pH values in vitro. CA has such distinctive CO2-catalyzing properties and is now being attended as prominent biocatalysts for CO2 sequestration technology development. Here, new genes of functional alpha type-CAs (aCA) from marine resources were acquired and synthesized as codon-optimized forms for E. coli expression. We set up over-expression system of aCA in E. coli. host and prepare separation/purification (renaturation, if necessary) procedures to produce high-functional CA forms.

키워드

저자정보

  • Seung Pil PACK Dept. of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700.
  • Bashistha Kumar KANTH Dept. of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700.
  • Ki Ha MIN Dept. of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700.
  • Young Ha RYU Dept. of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700.
  • Ki Baek YEO Dept. of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700.
  • Eui Kyung JANG Dept. of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700.
  • Mi-Ran KI Dept. of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700.

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자료제공 : 네이버학술정보

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