earticle

영어

Silicatein involved in biosilification in sponge has a cathepsin L-like structure and catalyzes silica deposition in vitro under near neutral pH and ambient temperature conditions. In this study, we employed recombinant silicatein and silicatein-like cathepsin (SLC), a modified cathepsin-like protein from Nematostella vectensis as form of fusion proteins with GST. GST portion of the fusion protein form increases the production of a protein of interest (silicatein or SLC) and binds to GSH coated surface, leading to an efficient immobilization of silica forming proteins on surface via GST-GSH interaction. Silica forming proteins bound surface catalyzed the formation of silica layer in the presence of tetraethyl orthosilicate as substrate. During catalyst-mediated silification, green fluorescent protein (GFP) or horseradish peroxidase (HRP) can be efficiently immobilized on the silica surface. Immobilized GFP or HRP retained their activity and were released gradually. This biocompatible silica coating technique can be employed to prepare biomolecule-immobilized surfaces or matrixes, which are useful for development of biocatalytic, diagnostic and biosensing system, or tissue culture scaffolds.