원문정보
초록
영어
The unripe fruits of Rubus coreanus Miquel, called Bokbunja, have been used as an important traditional medical material in Northeast Asia such as Korea and China. For the purpose of the investigation of the cosmetic functionality of unripe R. coreanus Miquel fruits, their phenolics were extracted and fractionized with diverse organic solvents. Among five fractions (n-hexane, chloroform, ethyl acetate, n-butanol, and water), ethyl acetate fraction showed the highest levels of total phenolics, total flavonoids, and antioxidant capacity. Cytotoxicity test results revealed the crude phenolic extract up to 100 μg/mL efficiently reduced melanin content in B16F1 cells without any effect on cell viability. Chloroform fraction unlike other fractions showed no considerable cytotoxicity as its concentration increased. Bokbunja extract, especially chloroform fraction, containing high level of total phenolics, total flavonoids, and antioxidant capacity, inhibited melanogenesis without any effect on cell viability. Therefore, the results above suggest that unripe R. coreanus Miquel fruits might be a functional material as a potent antioxidative and anti-melanogenic source.