원문정보
초록
영어
Two mushrooms of Sparassis crispa and Mycoleptodonoides atichisonii and wheat sprout (Triticum aestivum L. (keumkang)) were treated with 80% ethanol at room temperature for 72 hours. They were then extracted using ethyl acetate and n-butanol layer. All the fractions were loaded on the open column (one liter for each fraction) and analyzed by thin layer chromatography (TLC). The carbohydrate fractions were detected using chromogenic reagent and concentrated by evaporator. The separated fractions from mushrooms were characterized for the toxicity by MTT assay and measured for antitumor activity. The fractions from wheat sprout were evaluated for the toxicity and anti-inflammation activity by MTT/NO assay, using HPLC/MS. As a result, No. 2, 7, 12, 14, 17, and 23 fractions from wheat sprout showed higher anti-inflammation activity than control.