earticle

영어

Nanoencapsulation of thiamine dilauryl sulfate (TDS) was proved to effectively inhibit the spore germination of Fusarium oxysporum f. sp.raphani (F. oxysporum), as well as mycelial growth. The average diameter of nanoparticles was measured as 136 nm by being encapsulated with an edible encapsulant, lecithin, whose encapsulation efficiency was about 55% in containing 200 ppm of TDS concentration. The 100 ppm TDS nanoparticle solution showed better anti fungal activities as 59% inhibition of mycelial growth, compared to TDS solution, 100 ppm as 38%. These results were about similar or even better than the cases of treating 100 ppm of dazomet, a positive antifungal control (64%). Moreover, kinetic analysis of inhibiting spore germination were estimated as 6.6% reduction of spore germination rates after 24 h treatment, which were 3.3% similar to the case of treating 100 ppm of a positive control (dazomet) for the same treatment time. It was also found that TDS itself could work as an antifungal agent by inhibiting both mycelial growth and spore germination, even though its efficacy was lower than those of nanoparticles. Nanoparticles especially played a more efficient role in limiting the spore germination, due to their easy penetration into hard cell membranes and long resident time on the surface of the spore shell walls. It was possibly caused by that the nanoparticles were easily attached to the membrane of the cells and then well penetrated into the cell wall and long resident time on the surface of the spore shell walls. These results could tell that TDS can be effectively used as a bio-pesticide by being properly processed like nano encapsulation.