earticle

영어

The steaming process is most often used for processing fresh ginseng to make red ginseng, but its mechanism was not much studied yet. Therefore, in this work, Codonopsis laceolata was extracted under several extraction conditions with ethanol after being step-wise steamed such as the control, 70% ethanol extraction at 60℃ (CE), the extracts of one steaming (SCE1), three times steaming (SCE3) and five times steaming processes (SCE5). The highest amounts of nitric oxide from macrophage was measured as 39.34 pg/mL in adding 1.0 mg/mL of SCE5, and CE were shown the lowest secretion of nitric oxide. The production of prostaglandin E₂, also a inflammatory parameter, were observed as 1216, 1325, 1388 and 1530 pg/mL in adding 1.0 mg/mL of SCE5, SCE3, SCE1 and CE, respectively. The secretion of IL-6 and TNF-a form human B and T cells were also shown similar pattern to those of nitro oxide and PEE2, yielding 76.22 pg/mL and 90.22 pg/mL in adding SCE5. In general, higher steaming process could increase the immune activation activities possibly by relatively high concentration and elution of active components during the steaming processes. However, this process should well monitored and optimized to promote immune activation activities, not to generate the toxic substances of initiating the cancer cell growth like benzopyrene.