원문정보
초록
영어
In the present study, we characterized the YPD1 homologue of the thermotolerant methylotrophic yeast Hansenula polymorpha. The Ypd1 is an important phosphorelay protein in yeast two-component systems. The amino acid sequence of HpYpd1 shows 27.3% of identity to that of S. cerevisiae Ypd1. Deletion of HpYPD1 gene caused the cell sensitive to high concentrations of NaCl, sorbitol, and KCl. To understand the role of HpYPD1, transcriptome profiles of wild-type and YPD1 deletion mutant H. polymorpha strains challenged with 0.5 M NaCl for 30 min were analyzed. About 300 genes were differentially regulated in the wild-type and YPD1 deletion mutant strain. Interestingly, many genes involved in the osmotic stress response, general stress response, and cell surface and cell wall formation were up-regulated in wild-type strain. On the other hand, numerous genes involved in lipid metabolism were down-regulated in YPD1 deletion mutant strain. Our results indicate that YPD1 plays critical roles in osmotic stress response in H. polymorpha. Supported by the Intelligent Synthetic Biology Global Frontier Program, and the Next-Generation BioGreen 21 Program.