원문정보
초록
영어
In the present study, we identified the target gene of CpxAR twocomponent signal transduction system of the bovine rumen bacterium M. succiniciproducens. The recombinant CpxA sensor kinase protein was able to autophosphorylate and transphosphorylate the CpxR response regulator protein in vitro. Our previous study identified a putative 16-gene operon related to the cell wall and lipopolysaccharide biosynthesis as a putative CpxR target. The promoter region of the first gene, wecC encoding UDP-N-acetyl-D-mannosaminuronate dehydrogenase, was found to contain a sequence homologous to the CpxR box of E. coli. An electrophoretic mobility shift assay showed that the phosphorylated CpxR proteins were able to bind specifically to PCR-amplified DNA fragments containing the promotersequence of wecC. Furthermore, the real time PCR results with wild-type and cpxR mutant strains showed that wecC expression is regulated CpxR dependent manner. Our results suggest that the CpxRA system of M. succiniciproducens regulates cell wall biogenesis in response to envelop stress. Supported by the Intelligent Synthetic Biology Global Frontier Program, and the Next-Generation BioGreen 21 Program.