원문정보
초록
영어
The L-valine producing E. coli W strain was constructed by rational engineering. Engineering of E. coli for L-valine production has mostly been derived from E. coli K-12 strains. However, a high concentration of byproducts was observed during L-valine production in these strains, resulting in retarded cell growth and decreased product yield. E. coli W strain, which has high L-valine tolerance and produces byproducts at low level, was employed for the enhanced production of L-valine. The ilvA gene was deleted to eliminate the formation of 2-ketobutyrate, the intermediate precursor of L-isoleucine biosynthesis competing with L-valine biosynthesis. Next, L-valine biosynthetic genes, ilvBNmutandilvCED were plasmid-based amplified and positive-acting global regulator and a transporter were amplified. The final engineered strain was able to produce an impressively high concentration of L-valine up to 60.7g/L. [This work was supported by the Advanced Biomass R&D Center of Korea(2010-0029799) through Global Frontier Research Program of MEST. Further supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (NRF-2012-C1AAA001-2012M1A2A2026556)]