원문정보
초록
영어
Cadaverine is a five-carbon diamine that can be used as a building block for sustainable polymer production. Bio-based production of cadaverine from renewable feedstock is a promising and sustainable alternative to the petroleum-based chemical synthesis. Here, we report development of a metabolically engineered strain of Escherichia coli that produces cadaverine from glucose in defined medium. The pathways that lead to cadaverine degradation were blocked by genetic engineering. The engineered strain produced 9.61 g/L of cadaverine with a productivity of 0.32 g/L/h by fed-batch cultivation. [This work was supported by the Technology Development Program to Solve Climate Changes (systems metabolic engineering for biorefineries) from the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea (NRF-2012-C1AAA001-2012M1A2A2026556). Further support by the World Class University program (R32-2008- 000-10142-0) of the MEST are appreciated.]