earticle

영어

Malic acid is one of the commonly used chemicals. A Gram-negative facultative capnophilic rumen bacterium, Mannheimia succiniciproducens, produces mainly succinic acid using anaplerotic pathway under anaerobic condition. Wild type M. succiniciproducens strain does not produce malate. M. succiniciproducens has a merit for production of fumarate and malate production because it has strong anaplerotic pathway under CO2 conditions. Recently, our group published papers for developing the high succinate production strain by genome engineering. Based on this strain, the malate production strain was constructed. Especially, when the fumC gene encoding fumarase which converts malate to fumarate was deleted, this strain produced mostly malate instead of succinate. The results provide useful information for the rational metabolic engineering by optimizing metabolic fluxes to improve malate production in strains having a strong anaplerotic pathway such as rumen bacteria. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries. (NRF-2012-C1AAA001- 2012M1A2A2026556)]