원문정보
초록
영어
We constructed the novel pathway in Escherichia coli for the production of butanol by using L-valine as starting metabolite. Based on the previously constructed L-valine overproducing strain of E. coli, in which all the carbon flux towards L-valine formation was increased, the novel pathway and genes for the biosynthesis of butanol from 2-ketoisovalerate, the direct precursor of L-valine, was introduced. The resulting engineered E. coli strain was able to produce 118 mg/L butanol in batch fermentation. These results suggest that an efficient production of butanol is possible by properly assembling the synthetic metabolic pathways in E. coli. [This work was supported by the Advanced Biomass R&D Center of Korea (ABC-2010-0029799) through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST). Further supports by EEWS program of KAIST, and the World Class University program (R32-2008-000- 10142-0) of the MEST are appreciated.]