earticle

영어

Bio-based production of 1,5-diaminopentane from renewable feedstock is a promising and sustainable alternative to the petroleum-based chemical synthesis. Here, we report development of a metabolically engineered Corynebacterium glutamicum that produces 1,5-diaminopentane. L-lysine decarboxylase, which converts L-lysine directly to 1,5-diaminopentane, was amplified by plasmid-based overexpression of the cadA gene under the tac promoter in an industrial L-lysine producer C. glutamicum (U2 strain). However, the recombinant C. glutamicum (U2/pCEcadA) did not produce 1,5-diaminopentane and L-lysine was still detected in the culture medium. Thus, the cadA gene was modified using a codon adaptation program. With the redesigned cadA gene, we achieved 31.94 g/L of 1,5-diaminopentane without exogenous feeding of L-lysine by fed-batch fermentation of C. glutamicum (U2/pCEcadA). [This work was supported by the Technology Development Program to Solve Climate Changes (systems metabolic engineering for biorefineries) from the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea (NRF-2012-C1AAA001- 2012M1A2A2026556)]