원문정보
초록
영어
Biorefinery is leading to a new manufacturing paradigm for the industrial synthesis of chemicals from renewable biomass. However, one of the challenges in the biorefinery is a low-yield conversion process. In this study, the native redox cofactor regeneration system in Escherichia coli was engineered to implement non-native synthetic pathway for production of butyric acid. Butyric acid and its derivatives are utilized in various industrial products, and direct hydrogenation of butyric acid by copper-based catalysts can produce 1-butanol, which is a chemical in the world spotlight as a replacement for gasoline. The engineered strain JHL26, which regenerates NAD+ from NADH using butyrate as the only final electron acceptor enabled high-yield production of butyric acid from glucose (83.4% of the molar theoretical yield) with a high selectivity (butyrate/acetate ratio of 41). Furthermore, the high titer could be obtained through dual-phase extractive fermentation. This study represents dramatically improved industrial potential and could be broadly utilized for the production of various other useful chemicals in the fields of metabolic engineering and synthetic biology.