earticle

영어

Recently, the genetic modified organism has been used to produce antibiotics, bio-energy and antibodies. In this study, a non conventional yeast Kluyveromyces marxianus (KM) was employed to produce bioplastic precursors. For bioplastic production, KM was engineered to produce hexanoic acid from sugar such as glucose or galactose. Hexanoic acid has been commercially used to precursor for the synthesis of bioplastic. Beta – thiolase I (bktB), beta - thiolase II (phbA), acetyl-CoA acetyltransferase (atoB), 3-hydroxybutyryl-CoA dehydrogenase (hbd), crotonase (crt) and trans-enoyl-CoA –reductase (ter) were introduced to convert sugar to hexanoic acid. Titer of hexanoic acid was 150 mg/L – 354 mg/L at batch cultures. However, the produced hexanoic acid was decomposed very fast. Thus, atoB gene involved in the beta-oxidation reaction was replaced with malonyl - CoA carrier protein gene (MCT1) to increase product titer. In addition to gene regulation, new biosynthesis pathway constructed to increase hexanoic acid titer instead of original biosynthesis pathway.