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영어

Myrecetin, one of the flavonols having high antioxidant activity, was synthesized by multi enzyme reaction. The combinatorial synthesis of myrecetin was carried out using naringenin as a starting substrate in the Escherichia coli cell factory. To produce such valuable natural flavonol in large quantity, various plant biosynthetic pathways were employed. Primarily, dihydrokaempferol synthesis was attained by feeding naringenin using flavonol synthase (FLS) from Arabidopsis thaliana. Dihydrokaempferol was further converted to kaempferol by Flavanone- 3-hydroxylase from Arabidopsis thaliana and the formation of kaempferol was 12 mg/liter. The final B-ring hydroxylation step was catalyzed by flavonoid 3’,5’-hydroxylase (F3’5’H) known as cytochrome p450. To enhance the f3’5’H activity and expression level in E.coli host, transmembrane binding N-terminal region was genetically modified. The results in this study further suggest possibilities to produce mass scale flavonols using sustainable enzymatic methods.