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논문검색

Propionyl-CoA dependent biosynthesis of 2- hydroxybutyrate containing polyhydroxyalkanoates in recombinant Escherichia coli

원문정보

Si Jae PARK, Bong Keun SONG, Jonggeon JEGAL, Young Hoon OH, Jung Eun YANG, Seung Hwan LEE, Sang Yup LEE

한국생물공학회 한국생물공학회 학술대회 2013 춘계학술대회 2013.04 pp.225-225
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

We have previously reported in vivo biosynthesis of polylactic acid (PLA) and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] employing recombinant Escherichia coli strains by the introduction of evolved Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1Ps6-19). Using this in vivo PLA biosynthesis system, we engineered E. coli to synthesize PHAs containing 2-hydroxybutyrate (2HB) monomer from glucose. A heterologous metabolic pathway for the generation of 2HB from propionyl-CoA was constructed. Conversion of 2-ketobutyrate into 2HB was mediated by 2HB dehydrogenase encoded by the panE gene. Recombinant E. coli strain expressing the phaC1437, pct540, and prpE genes together with the panE gene successfully produced PHAs consisting of 2HB, 3HB, and a small fraction of lactate by varying the 3HB and propionic acid concentrations in the culture medium.

키워드

저자정보

  • Si Jae PARK Department of Environmental Engineering and Energy, Myongji University, Yongin-si, Gyeonggido 449-728.
  • Bong Keun SONG Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology,Daejeon 305-600.
  • Jonggeon JEGAL Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology,Daejeon 305-600.
  • Young Hoon OH Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology,Daejeon 305-600.
  • Jung Eun YANG Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701.
  • Seung Hwan LEE Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology,Daejeon 305-600.
  • Sang Yup LEE Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701.

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