earticle

영어

Bacterial cell surface display allows peptides or proteins to be displayed on the cell surface by fusing them with anchoring motifs. It can be used for numerous biotechnological applications including live vaccine development, screening-displayed peptide libraries, bioadsorbents, whole-cell biocatalysts, and biosensor development. Here, Escherichia coli outer membrane proteomes were analyzed to identify potential candidates for applying anchoring motifs. Of many proteins identified, the utility of outer membrane protein Y as an anchoring motif was examined. SDS-PAGE, Western blot, and whole-cell enzyme activity measurement confirmed the successful expression of fusion proteins on the surface of E. coli. Our results suggest that protein Y could be used as an anchoring motif of E. coli for displaying active enzymes and this system could be employed to various biocatalytic applications. [This work was supported by the Basic Science Research Program (2010- 0008826) and Converging Research Center Program (2009-0093652) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology]