earticle

영어

Typically, subcellular fractionation has been used for in-depth proteome analysis. This technique has the added benefits of reducing sample complexity, identifying additional unique proteins, localizing newly discovered proteins to specific organelles, and in some cases, allowing functional validation. In contrast to membrane proteome fractionation, periplasmic proteins have been yet well-fractionated. Here a more optimized method of periplasmic fractionation was developed. Highresolution periplasmic proteome reference maps of two E. coli strains, B and K-12, were constructed and compared. A total of 137 proteins, 69 proteins from the MG1655 gels and 71 proteins from the E. coli BL21(DE3) gels, were unambiguously identified with LC-MS/MS. The differences of periplasmic proteins between two strains will be discussed. [This work was supported by the Basic Science Research Program (2010-0008826) and Converging Research Center Program (2009-0093652) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology]