earticle

영어

Cephalosporin C, one of antibiotics was produced fromAcremonium chrysogenum M35 using xylose as a carbon source which could be obtained from lignocellulosic biomass. To produce cephalosporinC, three precursors of L-α-aminodipic acid, L-valine and L-cysteine are needed. L-α-Aminodipic acid and L-valine amounts are relatively abundant because they are major metabolites, but L-cysteine is limited and synthesized from stimulation material of methionine through reverse trans-sulfuration pathway though there is autotrophic cysteine production pathway from sulfate. In this study, cystathionie γ-lyase activity assay was utilized to confirm whether A.crysogenum M35 take the autotrophic pathway to produce a metabolite of cysteine without methionine. Cephalosporin C was reached maximum at 5 days of culture time, and activity assay was performed from 5 days cultured cell. When 8% of xylose was employed, cystathionine γ-lyase activity was found to be about 3.42 of OD value and 50.28 of specific OD value, and it is higher than the sample which employed 6% of glycerol for carbon source (approx. 35.06 of OD value). Also when 4% of xylose and 4% of glycerol was used as carbon source, approximately 50.32 of OD value was confirmed.