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Cloning of the Xylose Reductase Gene form Sour- Dough Yeast Candida milleri

원문정보

Hyoun-Soo SIM, Eun-Hee PARK, Se-Young KWON, Sang-Gi CHOI, Myoung-Dong KIM

한국생물공학회 한국생물공학회 학술대회 2013 춘계학술대회 2013.04 pp.222-222
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초록

영어

The entire nucleotide sequence of the xylose reductase (XR) gene in sourdough yeast Candida milleri CBS8195 was determined by degenerate polymerase chain reaction (PCR) and genome walking. Sequence analysis revealed an open-reading frame of 981 bp, encoding of 326 amino acids with a predicted molecular mass of 82 kDa. The derived amino acid sequence of C. milleriXR was 64.7% homologous to that of Kluyveromyces lactis. The cloned XR gene was expressed in a Δgre3 mutant of Saccharomyces cerevisiae and the recombinant strain was able to utilize xylose to produce 3.8 g/L of xylitol from 20 g/L xylose. An enzyme activity assay and semiquantitative reverse transcription PCR revealed that the expression of the CmXR is induced by xylose and repressed by glucose. The GenBank Accession No. for CmXR is KC599203.

키워드

저자정보

  • Hyoun-Soo SIM Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Eun-Hee PARK Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Se-Young KWON Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Sang-Gi CHOI Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Myoung-Dong KIM Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701.

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